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cd27 pe  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd27 pe
    Cd27 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd27+pe/CD274+(B7-H1)+Antibody%2C+anti-human%2C+REAfinity/pmc12969026-254-44-47
    Average 94 stars, based on 14 article reviews
    cd27 pe - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Recombination junctions from antibody isotype switching classify immune and DNA repair dysfunction
    Article Snippet: .. The following antibodies were used for staining: CD27-PE (dilution: 1:170, Miltenyi Biotec, #130-114-156, clone M-T271); IgD-PE-Cy7(dilution: 1:80, Miltenyi Biotec, #130-098-584), IgM-AF488(dilution: 1:1000, Life Technologies, #A21215); IgG-AF647(dilution: 1:500, Dianova, #109-606-170); IgA-AF647 (dilution: 1:500, Dianova, #109-606-01); IgA-APC-Vio770 (dilution: 1:170, Miltenyi Biotec, #130-113-473, clone IS11-8), CD19-Brilliant Violet 605 (dilution: 1.160, Becton Dickinson, #562653); CD20-PerCP-Vio700 (dilution: 1:170, Miltenyi Biotec, #130-113-377, clone LT20). ..

    Article Title: Recombination junctions from antibody isotype switching classify immune and DNA repair dysfunction.
    Article Snippet: .. The following antibodies were used for staining: CD27-PE (dilution: 1:170, Miltenyi Biotec, #130-114-156, cloneM-T271); IgD-PE-Cy7(dilution: 1:80,MiltenyiBiotec, #130-098-584), IgM-AF488(dilution: 1:1000, Life Technologies, #A21215); IgG-AF647(dilution: 1:500, Dianova, #109-606-170); IgA-AF647 (dilution: 1:500, Dianova, #109-606-01); IgA-APC-Vio770 (dilution: 1:170, Miltenyi Biotec, #130-113-473, clone IS11-8), CD19-Brilliant Violet 605 (dilution: 1.160, Becton Dickinson, #562653); CD20-PerCP-Vio700 (dilution: 1:170, Miltenyi Biotec, #130-113-377, clone LT20). ..

    Article Title: Recruitment of plasma cells from IL-21-dependent and IL-21-independent immune reactions to the bone marrow
    Article Snippet: .. Enriched cells were incubated with Fc Blocking Reagent (Miltenyi Biotec) following manufacturer’s instructions and subsequently stained for 30 min at 4 °C with the following anti-human antibodies: CD3-FITC (UCHT1, DRFZ in-house, 1:10), CD14-VioBlue (TÜK4, Miltenyi Biotec, Cat. 130-113-152, 1:200), CD27-PE (MT271, Miltenyi Biotec, Cat. 130-113-630, 1:100) and CD38-APC (HIT2, BioLegend, Cat. 303510, 1:25). ..

    Article Title: Recruitment of plasma cells from IL-21-dependent and IL-21-independent immune reactions to the bone marrow.
    Article Snippet: .. Enriched cells were incubated with Fc Blocking Reagent (Miltenyi Biotec) following manufacturer’s instructions and subsequently stained for 30min at 4 °C with the following anti-human antibodies: CD3-FITC (UCHT1, DRFZ in-house, 1:10), CD14-VioBlue (TÜK4, Miltenyi Biotec, Cat. 130-113-152, 1:200), CD27-PE (MT271, Miltenyi Biotec, Cat. 130-113- 630, 1:100) and CD38-APC (HIT2, BioLegend, Cat. 303510, 1:25). ..

    Fluorescence:

    Article Title: In severe COVID-19, SARS-CoV-2 induces a chronic, TGF-β-dominated adaptive immune response
    Article Snippet: After the sample was labeled sequentially by DAPI (Roche, Cat. No. 10236276001, dilution 1:5000) and all antibodies of interest as described above, the experiment was completed. .. Fluorescence-coupled antibodies used: IgA2-PE (Clone REA995, Miltenyi, Cat. No.130-117-763, dilution 1:50); CD27-PE (Clone REA499, Miltenyi, Cat. No. 130-114-166, dilution 1:50); CD38-PE (Clone IB6, Miltenyi, Cat. No. 130-113-427, dilution 1:50) and IgA-PE (Clone IS11–8E10, Miltenyi, Cat. No. 130-114-002, dilution 1:50). ..

    Article Title: SARS-CoV-2 in severe COVID-19 induces a TGF-β-dominated chronic immune response that does not target itself
    Article Snippet: After the sample was labeled sequentially by DAPI (Roche, Cat. No. 10236276001, dilution 1:5000) and all antibodies of interest as described above, the experiment was completed. .. Fluorescence-coupled antibodies used: IgA2-PE (Clone REA995, Miltenyi, Cat. No. 130-117-763, dilution 1:50); CD27-PE (Clone REA499, Miltenyi, Cat. No. 130-114-166, dilution 1:50); CD38-PE (Clone IB6, Miltenyi, Cat. No. 130-113-427, dilution 1:50); and IgA-PE (Clone IS11-8E10, Miltenyi, Cat. No. 130-114-002, dilution 1:50). ..

    Recombinase Polymerase Amplification:

    Article Title: Antigen-binding affinity is a key determinant of the durable antitumor activity of CD5 CAR-T cells
    Article Snippet: For detection of cell surface-bound anti-CD5 recombinant scFv, 2 × 10 5 cells were incubated with 10 ng of mIgG2a-Fc-tagged anti-CD5 scFv protein (Y-Biologics, Republic of Korea), washed, and subsequently stained with AF647-conjugated anti-mIgG2a antibody (clone RMG2a-62, BioLegend). .. The following antibodies were used: BioLegend, CD5-PE (clone UCHT2), CD69-PE (clone FN50), LAG3-FITC (Clone 7H2C65), mIgG2a-AF647 (clone RMG2a-62), and streptavidin-AF647; BD Biosciences, CD4-V500 (clone RPA-T4), CD8-V450 (clone RPA-T8), αβ TCR-PE (clone IP26), and γδ TCR-BV421 (clone 11F2); Thermo Fisher Scientific, PD-1-PE (clone J105) and CD27-PE (clone O323); Miltenyi Biotec, TOX-APC (clone REA473), Vδ1-PE-Cy7 (clone REA173), and Vδ2-V500 (clone 123R3); Cell Signaling Technology, Myc-AF647 or PE (clone 9B11). ..

    Article Title: Antigen-binding affinity is a key determinant of the durable antitumor activity of CD5 CAR-T cells
    Article Snippet: For detection of cell surface-bound anti-CD5 recombinant 430 scFv, 2 x 105 cells were incubated with 10 ng of mIgG2a-Fc-tagged anti-CD5 scFv protein 431 (Ybiologics, Republic of Korea), washed, and subsequently stained with AF647-conjugated 432 anti-mIgG2a antibody (clone RMG2a-62, BioLegend). .. The following antibodies were used: 433 BioLegend, CD5-PE (clone UCHT2), CD69-PE (clone FN50), LAG3-FITC (Clone 7H2C65), 434 mIgG2a-AF647 (clone RMG2a-62) and Streptavidin-AF647; BD Biosciences, CD4-V500 435 (clone RPA-T4), CD8-V450 (clone RPA-T8), αβ TCR-PE (clone IP26), and γδ TCR-BV421 436 (clone 11F2); Thermo Fisher Scientific, PD-1-PE (clone J105) and CD27-PE (clone O323); 437 Miltenyi Biotec, TOX-APC (clone REA473), Vδ1-PE-Cy7 (clone REA173) and Vδ2-V500 438 (clone 123R3); Cell Signaling Technology, Myc-AF647 or PE (clone 9B11). ..

    Incubation:

    Article Title: Recruitment of plasma cells from IL-21-dependent and IL-21-independent immune reactions to the bone marrow
    Article Snippet: .. Enriched cells were incubated with Fc Blocking Reagent (Miltenyi Biotec) following manufacturer’s instructions and subsequently stained for 30 min at 4 °C with the following anti-human antibodies: CD3-FITC (UCHT1, DRFZ in-house, 1:10), CD14-VioBlue (TÜK4, Miltenyi Biotec, Cat. 130-113-152, 1:200), CD27-PE (MT271, Miltenyi Biotec, Cat. 130-113-630, 1:100) and CD38-APC (HIT2, BioLegend, Cat. 303510, 1:25). ..

    Article Title: Recruitment of plasma cells from IL-21-dependent and IL-21-independent immune reactions to the bone marrow.
    Article Snippet: .. Enriched cells were incubated with Fc Blocking Reagent (Miltenyi Biotec) following manufacturer’s instructions and subsequently stained for 30min at 4 °C with the following anti-human antibodies: CD3-FITC (UCHT1, DRFZ in-house, 1:10), CD14-VioBlue (TÜK4, Miltenyi Biotec, Cat. 130-113-152, 1:200), CD27-PE (MT271, Miltenyi Biotec, Cat. 130-113- 630, 1:100) and CD38-APC (HIT2, BioLegend, Cat. 303510, 1:25). ..

    Blocking Assay:

    Article Title: Recruitment of plasma cells from IL-21-dependent and IL-21-independent immune reactions to the bone marrow
    Article Snippet: .. Enriched cells were incubated with Fc Blocking Reagent (Miltenyi Biotec) following manufacturer’s instructions and subsequently stained for 30 min at 4 °C with the following anti-human antibodies: CD3-FITC (UCHT1, DRFZ in-house, 1:10), CD14-VioBlue (TÜK4, Miltenyi Biotec, Cat. 130-113-152, 1:200), CD27-PE (MT271, Miltenyi Biotec, Cat. 130-113-630, 1:100) and CD38-APC (HIT2, BioLegend, Cat. 303510, 1:25). ..

    Article Title: Recruitment of plasma cells from IL-21-dependent and IL-21-independent immune reactions to the bone marrow.
    Article Snippet: .. Enriched cells were incubated with Fc Blocking Reagent (Miltenyi Biotec) following manufacturer’s instructions and subsequently stained for 30min at 4 °C with the following anti-human antibodies: CD3-FITC (UCHT1, DRFZ in-house, 1:10), CD14-VioBlue (TÜK4, Miltenyi Biotec, Cat. 130-113-152, 1:200), CD27-PE (MT271, Miltenyi Biotec, Cat. 130-113- 630, 1:100) and CD38-APC (HIT2, BioLegend, Cat. 303510, 1:25). ..



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